The effect of acupunctural and moxibustional therapy on the apoptosis of breast cell MCF-7
- VernacularTitle:“针灸血清”对乳腺癌MCF-7细胞凋亡及细胞周期的影响
- Author:
Yan LI
;
Gang GUO
;
Bin LI
- Publication Type:Journal Article
- Keywords:
Acupuncture?Moxibustion?Breast Neoplasms?Apoptosis?Cell cycle
- From:
Chinese Journal of Current Advances in General Surgery
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect on the apoptosis of breast cancer cell MCF-7 with acupunctural and moxibustional therapy.Methods:Zusanli and Sanyinjiao neiguan in both sides of female Wistar mice were acupunctured and moxibusted.MCF-7 cell was cultirated with the serum.To detecte the cytoxicity with MTT,and detecte the apoptosic rate and Fas expression rate with flow cytometry after 72 hours.Results:The Fas expression was obviously increased upto 57.70% after 72h.The proliferation of MCF-7 was inhibited remarkably.The inhibition rate were 17.55%,24.71% and 31.85% after 12h,24h and 72h.The apoptosic rate were 8.72% and 10.18% after 72h,remarkably higher than the control group(P
