The effects of adramycin on the human liver hilar cholangiocarcinona cell line FRH-0201
- VernacularTitle:阿霉素对人胆管癌细胞株FRH-0201的生物学特性的影响
- Author:
Ming LI
- Publication Type:Journal Article
- Keywords:
Biliary tract neoplasms?Adramycin?Tumor cells,cultured?Cell cycle?Cell size?CA125?CA19-9
- From:Chinese Journal of Current Advances in General Surgery
2004;0(05):-
- CountryChina
- Language:Chinese
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Abstract:
Objective:To investigate the effects of adramycin on cell line of FRH-0201.Methods:Human hilar cholangiocarcinona cell line were cultured with adramycin (2?g/ml) through lasting different times,finally this cell line can survive after 72h cultured in the culture liquid with the adramycin(1?g/ml) .Then the medium was replaced with a fresh one without adramycin,and the cells were continuously cultured for 1 month.The in vitro studies included the observation of the appearance with optical microscope,MTT cell proliferation assay,analysis of the cell cycle by Flow cytometry(FACS),assays of tumor marker using en{yme-linked immunosor bent assay(ELISA),the fluorescence density of adramycin.Results:Compared to the parent FRH-0201cell line:The morphology showed typical morphological characteristics,the doubling time prolonged about 3h,the cell cycle by flow cytometry identified in G_1,G_2 and S phase of cell cycle were 40.50%,19.74%and 39.77% in trial group,and 69.83%,7.29%and 22.88% in parent group,respectively.Tumor marker has no difference between them,the fluorescence density of epirubicin descending 5.2 times.Conclusion:The adramycin can affect the cell cycle and cause the change of metabolism.
