The effect of ahylysantinfarctase on the growth of fetus lens epithelial cells in vitro
- VernacularTitle:蝮蛇抗栓酶对胎儿晶状体上皮细胞体外生长的影响
- Author:
Li QIN
;
Fang BAI
;
Xiaoheng LI
- Publication Type:Journal Article
- Keywords:
ahylysantinfarctase;
posterior capsule opacification;
fetal lens epithelial cell;
cell culture;
disintegrin
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of ahylysantinfarctase (AF) on the growth of fetus lens epithelial cells (LECs) in vitro. Methods Fetal lens anterior capsule membranes from the eyeballs of dead fetus delivered by induced labor underwent primary cell culture with the modified method. The second or third generations of fetal LECs were used in this experiment. They were exposed to AF of six kinds of concentrations (2?10~ -4 , 4?10~ -4 , 8?10~ -4 , 16?10~ -4 , 32?10~ -4 , 64?10~ -4 u/mL). Their morphologic changes were observed with the inverted microscope and the value of absorption (A) in each well was tested by MTT assay to examine the effect of AF on the growth of the fetal LECs collected. Results In the experimental groups, especially AF groups of higher concentration, LECs exhibited inordinate shape, less kytoplasm, more cellular endoparticles, wider intercellular space and random disposition, and even adherence failure. After the cells grew in AF of different concentrations for 48h, A value in each group decreased with the increase of AF concentration, and differed significantly between all the experimental groups (P
