Raised immune responses of HBV preS_2S DNA vaccines by administrating genetic adjuvant at different time
- VernacularTitle:基因佐剂注射时相对HBV preS_2S疫苗免疫作用的影响
- Author:
Hongmei CHEN
;
Xuefan BAI
;
Changxing HUANG
;
Guangyu LI
- Publication Type:Journal Article
- Keywords:
HBV DNA vaccine;
IL-2;
Genetic adjuvant
- From:
Chinese Journal of Immunology
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the different effect of fused gene IL-2/Fc administrated at different time on immune responses induced by HBV preS_2S genetic vaccine.Methods:BALB/c mice were immunized with purified recombinant plasmids at 0, 2, 4 weeks. Two groups were designed. One group were injected with pcDNA3.1S_2S at first, then pcDNA3.1IL-2/Fc was administrated after three days. While the other group were injected two plasmids at the same time. We compared the immune responses through detecting the anti-HBs titers, CTL cytotoxicity level, proliferation of lymphocytes and cytokines levels secreted by cultural splenocytes.Results:The data demonstrated that the humoral and cellular immune responses induced by pcDNA3.1IL-2/Fc as adjuvant and administrated after HBV preS_2S DNA vaccine 3 days mice were dominant higher than other groups.Conclusion:Genetic adjuvant administrated after Ag priming can enhance its effect.
