Th1 cellular immune response elicited by recombinant adenovirus transduced dendritic cells for HCV-NS3 in vivo
- VernacularTitle:HCV-NS3重组腺病毒转染树突状细胞体内诱导抗原特异性Th1应答的研究
- Author:
Ming XIANG
;
Encke JENS
- Publication Type:Journal Article
- Keywords:
HCV NS3;
Adenovirus;
Dendritic cells;
Th1 immune response
- From:
Chinese Journal of Immunology
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The aim of this study was to explore the vaccine potential of adenovirus transduced DC encoding for HCV nonstructural protein NS3 in a murine model.Methods:Dendritic cells were isolated from bone marrow of BALB/c mice and cultivated with cytokine cocktail for 7 days. Recombinant adenovirus was added to DC cultures at day 7,at a titration of 2?10~9 pfu/10~6 DC/well. FACS and Western blot techniques were used to identify the DC mature state and the protein expression, respectively. BALB/c mice were primed/boosted immunized with DC based vaccines at 3?10~5 cells for one mouse at a 10 day interval. 10 days after the second immunization, cytokines secretion and IFN-? generating T cells response were evaluated by ELISA and ELLISPOT analysis.Results:Our current data indicate that AdNS3 can stimulate the maturation of DC and efficiently express authentic HCV nonstructural proteins in infected cells. BALB/c mice inoculated with AdNS3 transduced DC twice generated strong IFN-? secreting T cells response(P
