Purification of SEB by McAb affinity chromatography and activity analysis
- VernacularTitle:单克隆抗体亲和层析法纯化SEB及其活性鉴定
- Author:
Jing ZHANG
;
Huifang WANG
;
Chuan GAO
;
Yunyang SONG
- Publication Type:Journal Article
- Keywords:
Monoclonal antibody;
Staphylococcal enterotoxin B;
Affinity chromatography
- From:
Chinese Journal of Immunology
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare highly purified Staphylococcal enterotoxin B(SEB) by affinity chromatography and test its activities.Methods:Anti-SEB McAb(1D2) purified by precipitation method with caprylic acid was coupled to Sepharose 4B. And then the SEB was isolated using an affinity chromatography column. In addition, we analyzed the superantigen activity and antigen activity of SEB.Results:The purification efficiency of SEB was 60.71% by affinity chromatography. Its purity was higher than those of standard preparation and the SEB purified by ion change chromatography. At the same time, the purified SEB by affinity chromatography possesses favourable activities of superantigen and antigen.Conclusion:McAb affinity chromatography could be used for purification of SEB with high efficiency.
