Washed platelets at low concentration promote the proliferation of human dental pulp cells
- VernacularTitle:低浓度洗涤血小板促进人牙髓细胞增殖的作用机制探讨
- Author:
Duan JIANMIN
;
Wang WEIJIAN
;
Kikuchi HIROTAKA
- Publication Type:Journal Article
- Keywords:
Human;
Dental pulp;
Cell;
Blood platelets;
Prostaglandin E_2;
Cyclooxygenase-2
- From:
Journal of Practical Stomatology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of washed platelets at low concentration(WPLT)on the proliferation of human dental pulp cells(HDPCs).Methods:WPLT was prepared from a male venous blood,cultured HDPCs were exposed to the WPLT at 50 ml/L,100 ml/L and 200 ml/L for 0.5～24 h respectively.Cell proliferation and prostaglandin E_2 production were examined by cell counting and Prostaglandin E_2 EIA Kit-Monoclonal respectively.Cyclooxygenase-2(COX-2)mRNA expression was determined by real time RT-PCR.Results:WPLT at 50 ml/L promoted HDPC proliferation and increased COX-2 mRNA expression at 3-hour-exposure.That at 50～200 ml/L increased the production of PGE_2 dose-depandently.Indomethacin or dexamethasone reduced WPLT-induced HDPC proliferation,and this inhibitory effect was reversed by exogenous PGE_2.PGE_2 promoted HDPC proliferation at 5-100 ng/ml.Conclusion:WPLT may induce prostaglandin E_2 production,thereby promote HPDC proliferation.
