The induction of apoptosis by epigallocatechin-3-gallate in xenograft nude mice with human gastric cancer cells
- VernacularTitle:表没食子儿茶素没食子酸酯诱导人胃癌细胞裸鼠移植瘤凋亡的研究
- Author:
Changying LI
;
Wei ZHOU
;
Juan CAO
;
Shaona ZOU
;
Zhaoyang LUO
- Publication Type:Journal Article
- Keywords:
eigallocatechin-3-gallate;
xenografts;
nude mice;
apoptosis;
therapy effect;
cultured tumor cells
- From:China Oncology
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Background and purpose:To investigate the induction of apoptosis by epigallocatechin-3-gallate(EGCG) in xenograft nude mice with human gastric cancer cells and its molecular mechanism. Methods:Human gastric cancer cells were planted into nude mice in order to establish the cancer model, the different dosages of EGCG were injected intraperitoneally in the nude mice. After treatment, flow cytometry (FCM) was used to detect the apoptosis of implanted tumor cells. Immunohistochemical staining was used to detect the expression of apoptosis-related genes like Bal-2 and Bax in implanted tumor.Results:EGCG significantly inhibited tumor growth after being injecting intraperitoneally in the nude mice. The apoptotic cells in implanted tumor could be detected by flow cytometry with PI staining. The expressions of Bax、Caspase-3 were upregulated and Bcl-2 expression was downregulated in implanted tumor.Conclusions:EGCG could significantly inhibit tumor growth in xenograft nude mice with human gastric cancer cells through inducing apoptosis. The down-regulation of Bcl-2 expression and up-regulation of Bax expression observed could result in the activation of Caspase-3, the pathway might account for the induction of apoptosis.
