Change of survivin gene expression in gastric cancer cell line BGC-823 induced by hydroxycamptothecine
- VernacularTitle:Survivin在羟基喜树碱诱导胃癌细胞株BGC-823凋亡过程中的变化及其意义
- Author:
Xuan CHEN
;
Ziwei WANG
- Publication Type:Journal Article
- Keywords:
survivin;
gastric neoplasm;
hydroxycamptothecine;
chemotherapy tolerance
- From:
China Oncology
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Background and purpose:The antitumor drugs produce a marked effect through the induction of apoptosis. The resistance to chemotherapy has been found to be associated with abnormal expression of genes related to apoptosis. We designed this experiment to investigate the role of survivin gene in the resistance to chemotherapy. Methods:After being treated with Hydroxycamptothecine (OPT,21.96mmol/L), the apoptosis in gastric cancer cell line BGC-823 cells were evaluated by either flow cytometry(FCM) or the terminal deoxynucleotidy transfer mediated dUTP-biotin nick end labeling(TUNEL). The expression of Survivin was detected by reverse transcription-Polymerase chain reaction(RT-PCR) and immunohistochemistry.Results:Compared to control group, the OPT can induced apoptosis in BGC-823[(12.68?0.17)% vs (3.35?0.13)% by FCM,(16.00?1.23)% vs (2.78? 0.84)% by TUNEL[, OPT could upregulate survivin expression in BGC-823 cells (1.489?0.041)% vs (0.756?0.037)% in terms of level of mRNA,(0.928?0.046) vs (0.303?0.032) in terms of protein expression).Conclusions:The survivin gene may be associated to the resistance to chemotherapy through the induction of gene expression.
