Analysis and improvement of methylation-specific polymerase chain reaction
- VernacularTitle:甲基化特异性PCR技术的分析及改良
- Author:
Shaohui YANG
;
Dongqiu DAI
- Publication Type:Journal Article
- Keywords:
Methylation-specific polymerase chain reaction
- From:
Cancer Research and Clinic
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze and improve Methylation-specific Polymerase Chain Reaction. Methods Methylation state of DAPK in gastric cancer was detected by this improved MSP. Results 100 % of frozen tissues and 24.1% of paraffin-embedded tissues could get either methylated production or unmethylated production. Conclusion Improved MSP is a convenient, sensitive and specific way to screen DNA methylation, especially for frozen tissues, but for paraffin-embedded tissues,it may be unsuitable.
