Expression and Functional Significance of Lung Resistance Protein in Pancreatic Adenocarcinoma Cell
- VernacularTitle:肺耐药蛋白在胰腺癌细胞中的表达及意义
- Author:
Junchao GUO
;
Changqing YAN
;
Quan LIAO
;
Yupei ZHAO
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasm Multidrug resistance Acquired drug resistance
- From:
Chinese Journal of Bases and Clinics in General Surgery
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression of lung resistance protein (LRP) and investigate its significance in pancreatic carcinoma cell lines (SW1990, PCT-2, PCT-3, PCT-4, Aspc-1, Capan-1, Mia-PaCa-2 and Panc-1). Methods Reverse transcription PCR (RT-PCR) and immunocytochemistry (ICC) were carried out to investigate the expression of LRP. Results LRP mRNA was absent in PCT-2 cell line by RT-PCR. Mild to moderate expression level was found in other pancreatic carcinoma cell lines. PCT-4, Aspc-1 and Panc-1 presented the highest LRP mRNA expression level, in contrast, SW1990, PCT-3, Capan-1 and Mia-PaCa-2 showed moderate LRP mRNA expression. The median value was 0.56?0.33. LRP was further validated by ICC. Absent to weak protein expression of LRP was found in PCT-2 and PCT-3. Overexpressed LRP was present in SW1990, Capan-1 and Aspc-1, furthermore, the highest expression of LRP was found in Panc-1, Mia-PaCa-2 and PCT-4 cell lines. Conclusion All these data showed that LRP might play an important role in multidrug resistance of pancreatic carcinoma.
