Expression of Anti-Tumor-Associated Glycoprotein-72 Single Chain Variable Fragment in Human Breast Cancer Cells
- VernacularTitle:抗肿瘤相关糖蛋白-72单抗在人乳腺癌中的表达
- Author:
Hongyong XU
;
Li XU
;
Kaizong LI
;
Jianhong GAO
- Publication Type:Journal Article
- Keywords:
Breast cancer Tumor associated glycoprotein-72 Single chain variable fragment
- From:
Chinese Journal of Bases and Clinics in General Surgery
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of tumor associated glycoprotein-72 (TAG-72) in human breast cancer cells. Methods Two breast cancer cell lines MCF-7 and Bcap-37 were cultured and prepared. TAG-72 expressions in MCF-7 and Bcap-37 were detected immunochemically with anti-TAG-72 single chain variable fragment (scFv). Results TAG-72 was positively expressed in MCF-7 cells but negatively expressed in Bcap-37 cells. Conclusion Tumor associated antigen TAG-72 is expressed in certain human breast cancer cells, which indicate TAG-72 may be used as a tumor maker and anti-TAG-72 scFv may play a role in the diagnosis and treatment of breast cancer.
