The effect of La protein on replications of HBV in cultured cell
- VernacularTitle:细胞内La表达对乙肝病毒复制的影响研究
- Author:
Hui ZHANG
;
Jinghui SUN
;
Honglian GENG
;
Gaolin LIU
;
Xiantao KONG
;
Longyi TAN
- Publication Type:Journal Article
- Keywords:
La protein;
SiRNA;
HBV
- From:
Chinese Journal of Immunology
2000;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prove whether La protein could effect the replications of the HBV in cultured cells.Methods:Three specific SiRNAs for human La protein was obtained by transcription in vitro. After transfection with the SiRNAs into HepG2.2.15 cell, the levels of La protein mRNA and HBV DNA were detected by real-time PCR.Results:The HBV DNA secreted by the cell transfected with the SiRNAs was reduced with reduction of the mRNA of the La protein.Conclusion:The La protein can protect the replications of the HBV in cultured cells.
