Promoting the proliferation of bone marrow mesenchymal stem cell by recombinant human granulocyte colony-stimulating factor
- VernacularTitle:重组人粒细胞集落刺激因子促进骨髓间充质干细胞增殖
- Author:
Qihuan LIU
;
Fanjun CHENG
;
Qingping GAO
- Publication Type:Journal Article
- Keywords:
Granulocyte colony stimulating factor,recombinant;
Mesenchymal stem cells;
Cell cycle;
Cell proliferation
- From:
Chinese Journal of Organ Transplantation
2005;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects on proliferation of bone marrow mesenchymal stem cells (MSCs) by recombinant human granulocyte colony-stimulating factor in mice. Methods Kunming mice were randomly divided into G-CSF and control groups (n=15). The mice were subjected to subcutaneous injections of rhG-CSF at a dose of 80 ?g/kg per day and control of saline for 5 days. The bone marrow was obtained on 6th, 12th and 168th h respectively after the final administration. The MSCs were separated and cultured, and the colony-forming unit-fibroblast (CFU-F) was evaluated. The cell cycle and the surface antigens were analyzed by flow cytometry. Results The number of CFU-F was increased after administration of the rhG-CSF (P 0.05). Flow cytometic detection of MSCs surface marks in fibroblast colony showed CD34~ -, CD133~ -, CD90~ + and CD105~ +, with the percentage of 2.5 %, 3.1 %, 67.0 % and 78.0 %, respectively. After mobilization with rhG-CSF, the percentage of G_0/G_1 phases in bone marrow MNCs was decreased (P
