The effect of atorvastatin on the expression and activity of matrix metallproteinases in rabbit abdominal arterial atherosclerosis plaque
- VernacularTitle:阿托伐他汀对家兔动脉粥样硬化斑块中基质金属蛋白酶的影响
- Author:
Yulin WANG
;
Xin ZHAO
;
Jifeng YAN
;
Xiangjun YANG
;
Zhihua LIU
- Publication Type:Journal Article
- Keywords:
Atherosclerosis;
Matrix metalloproteinase;
Atorvastatin
- From:Chinese Journal of Geriatrics
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of atorvastatin on the expression and activity of matrix metalloproteinases (MMPs) in rabbit abdominal arterial atherosclerotic plaque. Methods Eighteen male New Zealand rabbits weighing 2 kg were randomized to normal control group (n=6) and hypercholesterolemia group (n=12). The latter was given hypercholesterol diet for 2 weeks, and then catheter-induced abdominal aortic wall injury was performed. Rabbits in hypercholesterolemia and aortic injury group were randomized to model group (n=6,4 weeks of hypercholesterol diet) and atorvastatin(5 mg?kg-1?d-1 for 4 weeks)group (n=6). Finally, the levels of MMP2 and MMP9 protein and mRNA in the abdominal aortic artery were measured by immunohistochemical analysis, reverse transcription-polymerase chain reaction (RT-PCR), sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) zymography. Results The intimal-medial thickness[(0. 49?0. 072) mm vs (0.66?0.079) mm, P
