Research on apoptosis mechanism of HT-29 colon cancer cell induced by NDGA
- VernacularTitle:脂氧合酶抑制剂NDGA对HT-29结肠癌细胞诱导凋亡机制研究
- Author:
Guangtao XIA
;
Yuanchao ZHANG
;
Sensen WU
;
Lixia YIN
- Publication Type:Journal Article
- Keywords:
Lipoxygenase inhibitors?Apoptosis?5-Lipoxygenase?Telomerase?Calcium?Colonic neoplasms
- From:
Chinese Journal of Current Advances in General Surgery
1999;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To find out the apoptosis mechanism of HT-29 colon cancer cell induced by NDGA,the lipoxygenase inhibitor in vitro.Methods:We applied respectively:RT-PCR to detect colon cancer cell’s expression of 5-LOX messenger ribonucleic acid(5-LOXmRNA) and that of messenger ribonucleic acid about human telomerase reverse transcriptase(hTERTmRNA)respectively,confocal laser scanning electron microscope to examine intracellular free calcium.Results:Colon cancer cell lines HT-29 showed positive expression of 5-LOXmRNA.This expression became weaker following the rise of cell’s apoptosis and so were hTERTmRNA.Intracellular Free calcium increased following the rise of cell’s apoptosis.Conclusion:The apoptosis of tumor cell is caused by a combination of factors,with 5-LOX,telomerase and free calcium all active in the course.
