Soluble expression of human carboxypeptidase A1 active center in pichia pastoris
- VernacularTitle:人羧肽酶A1的毕赤酵母可溶表达
- Author:
Jing YI
- Publication Type:Journal Article
- Keywords:
carboxypeptidase A1;
pichia pastoris;
gene expression
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1982;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
(Objective)To express the human carboxypeptidase A1 in soluble form in pichia pastoris.(Methods)Human carboxypeptidase A1 gene was amplified from pGEX-CPA1 recombinant plasmid and subcloned into pPIC9K yeast expression vector.Recombinant vector pPIC9K-CPA1 was transformed into pichia SMD1168 by electroporation.Positive recombinant yeast strains were screened through nutrition defect and G418 resistance.Expression of the integrated multicopy recombinant yeast strains were induced.Results The yeast recombinant expression vector pPIC9K-CPA1 was constructed.Recombinant yeast strains which integrated over 16 recombinant vector copies and soluble human carboxypeptidase A1 protein were obtained.(Conclusion)The human carboxypeptidase A1 can be expressed successfully in pichia pastoris,which will be useful for further research on ADEPT of prospate.
