Construction and identification of fusion gene of NT4-p53(N15)-Ant
- VernacularTitle:NT4-p53(N15)-Ant融合基因的克隆和鉴定
- Author:
Liping SONG
;
Yueping LI
;
Shudong QIU
;
Guangxiao YANG
;
Quanying WANG
- Publication Type:Journal Article
- Keywords:
p53(N15)-Ant;
NT4 signal peptide;
gene clon;
carcinoma
- From:Journal of Xi'an Jiaotong University(Medical Sciences)
1982;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct the expression box of fusion gene NT4-p53(N15)-Ant.Methods The gene of p53(N15)-Ant was obtained by PCR of two primers templating with each other and T-vector cloning method.The positive clone was identified and analyzed by the restriction enzymes and sequencing respectively.After digested with restriction enzyme,the interest gene of p53(N15)-Ant was subcloned into the plasmid pBV220/NT4.Results The gene of p53(N15)-Ant was confirmed by the digestion of restriction enzyme and sequencing.The recombinant plasmid pBV220/NT4p53(N15)Ant was identified by the digestion of restriction enzyme and agarose gel electrophoresis and the results conformed theoretical values.Conclusion The plasmid pBV220 containing the expression box of NT4-p53(N15)-Ant was successfully constructed by molecular cloning and recombination techniques in vitro,which will guide further study on gene therapy of cancer.
