Study of loss of imprinting of insulin-like growth factor Ⅱ and H19 genes in cervical carcinoma
- VernacularTitle:印记基因H19和胰岛素样生长因子Ⅱ在人子宫颈癌组织中印记缺失及意义
- Author:
Yueling WANG
;
Xinyuan YANG
;
Xu LI
- Publication Type:Journal Article
- Keywords:
cervical carcinoma;
insulin-like growth factor Ⅱ;
H19;
loss of heterozygosity;
loss of imprinting
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1982;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the loss of imprinting(LOI) of insulin-like growth factor Ⅱ(IGF2) and H19 genes in cervical carcinomas.Methods Polymerase chain reaction(PCR) method was used on DNA samples extracted from 40 cases of cervical carcinoma and 20 cases of normal control tissues to detect the heterozygosity of IGF2 and H19 genes.The LOI of IGF2 and H19 genes was detected by RT-PCR.Results The heterozygous frequency of IGF2 was 52% in cervical cancer samples.Comparatively,in 20 matched normal samples,13 cases showed the heterozygosity(65%).The biallelic expression of IGF2 was detected in 10 cases among the 21 informative cervical cancer samples(47.6%),however,1 case in 13 heterozygous samples of control group(7.7%)((P0.05).Conclusion LOI and LOH of IGF-2 and H19 genes are involved in cervical carcinomas.LOI of IGF2 and H19 may be involved in the initiation stage of carcinogenesis of cervical carcinoma.
