Effects of p27mt gene transfection mediated by replication deficient adenovirus on the proliferation and apoptosis of human hepatocellular carcinoma cells
- VernacularTitle:重组腺病毒介导突变型p27基因转移对肝癌细胞SMMC-7721生长的影响
- Author:
Bing XU
;
Xianxiang CHEN
;
Feng YU
;
Qinghe CAI
;
Du ZHANG
- Publication Type:Journal Article
- Keywords:
p27mt gene transfection;
Adenovirus;
Cell proliferation;
Apoptosis;
Human hepatocellular carcinoma cells
- From:
Cancer Research and Clinic
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the effects of p27mt gene transfection on the proliferation and apoptosis of human hepatocellular carcinoma cell (HCC) lines SMMC-7721. Methods A replication deficient adenovirus vector encoding p27mt (Ad-p27mt) was used and p27mt cDNA was transfected into human SMMC-7721 cell lines in vitro. The synthesis of DNA in SMMC-7721 cells was determined by using 3H-thymidine incorporation; the cell apoptosis was determined by flow cytometry, TUNEL method and DNA fragmentation analysis. Results The virus titer was 7.95?1012 cfu/ml, the transduction efficiency was 100 % when multiplicity of infection ≥50, FCM analysis revealed a sub-G1 cell peak in Ad-p27mt transduced hepatocellular carcinoma cell lines. Agarose electrophoresis showed marked ladder .The difference of apoptotic index between the Ad-p27mt group and the control group was statistically significant (58.6?4.3, vs 4.5?1.6, P
