Relationship of cytokines and cytokine receptors gene polymorphisms with acute rejection in kidney transplantation recipients
- VernacularTitle:细胞因子及受体基因多态性与移植肾急性排斥反应的关系
- Author:
Tieming LU
;
Jianming TAN
- Publication Type:Journal Article
- Keywords:
Cytokines;
Receptors,cytokine;
Graft rejection
- From:
Chinese Journal of Organ Transplantation
2005;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship of cytokines and cytokine receptors gene polymorphism with acute rejection in kidney transplantation recipients,whose 21 single nucleotide polymorphisms in 5 kinds of cytokines and their receptors were tested with cytokine oligonucleotide array.Methods According to the allele sequences of 21 gene polymorphisms of IL-4,IL-6,IL-10,TNF-?,TGF-?_1 and their receptors,58 oligonucleotide probes were synthesized. A pair of group special primers labeled by the Cy5 were designed and were used in the PCR. The labeled PCR products with Cy5 were hybridized with array. The signals were scanned by a scanner and analyzed by image software. Genomic DNA samples from the peripheral blood lymphocytes of 144 kidney transplant recipients were tested by this array. The distribution of 21 single nucleotide polymorphism in cytokines and cytokine receptors was compared between two groups according to the presence or absence of acute renal rejection.Results In recipients,the gene polymorphism distribution in rejection group and non-rejection group showed significant difference (P
