Application of dual-color interphase fluorescence in situ hybridization in sex-mismatched hematopoietic stem cell transplantation
- VernacularTitle:双色间期荧光原位杂交技术在异性间造血干细胞移植中的应用
- Author:
Run ZHANG
;
Jianyong LI
;
Jinlan PAN
- Publication Type:Journal Article
- Keywords:
In situ hybridization,fluorescence;
Hematopoietic stem cell transplantation;
Neoplasm,residual
- From:
Chinese Journal of Organ Transplantation
2005;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the significance of dual-color interphase fluorescence in situ hybridization (FISH) in the engraftment estimation and minimal residual disease (MRD) monitoring after sex-mismatched allogeneic hematopoietic stem cell transplantation (allo-HSCT).Method The fluorescence signal of samples from 35 cases in different periods after sex-mismatched allo-HSCT were detected by interphase FISH using chromosome enumeration probes (CEP) X and Y. Results All of 35 patients had been determined to obtain engraftment after allo-HSCT. When the disease relapsed,FISH showed that the percentage of donor chromosomes was decreased and when the disease got remission,the percentage of donor chromosomes increased. When conventional cytogenetics showed 100 % XX or 100 % XY,FISH showed different percentage of host chromosomes.Conclusions The test of dual-color interphase FISH is reliably sensitive and simple for engraftment evaluation and MRD monitoring post HSCT. It is a good complement method to cell morphology and traditional karyotype analysis.
