Injury to hepatocytes and sinusoidal endothelial cells caused by cold preservation of donor liver after transplantation in rats
- VernacularTitle:大鼠供肝冷保存时间延长损伤移植肝肝细胞和肝窦内皮细胞
- Author:
Jin ZHU
;
Jiahong DONG
;
Ping CHEN
- Publication Type:Journal Article
- Keywords:
Liver transplantation;
Hepatocytes;
Endothelial cells;
Rat
- From:
Chinese Journal of Organ Transplantation
2005;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the primary target of injury resulting from cold preservation/reperfusion after liver transplantation in rats. Methods Male SD rats were divided into three groups randomly: sham group,UW 1-h group and UW 12-h group. Orthotopic liver transplantation was performed by using the technique described by Kamada with a modification. Survival rate of rats within 168 h postoperation was observed. Liver tissue specimens and blood samples were collected at 8 time points predetermined as 1,6,12,24,48,72,96 and 168 h postoperation. Six animals were used per time-point. The liver function was evaluated by serum ALT,AST and HA levels. Morphology was observed under light microscopy and TEM. Additionally,the incidence of apoptosis (AI) in hepatocytes and sinusoidal endothelial cells (SECs) were measured separately by TUNEL method.Results The survival rate at 168 h in UW 12-h group was 50 %,significantly lower than that in UW 1-h group (F= 6.39 ,P
