Repair of rabbit cartilage defects by composite of cocultures of autogenic bone marrow-derived mesenchymal stem cells and chondrocytes with allogenicfully deproteinized bone
- VernacularTitle:自体骨髓间充质干细胞和软骨细胞共培养复合同种异体完全脱蛋白骨修复关节软骨全层缺损的研究
- Author:
Yayi XIA
;
Wanwen FENG
;
Zhengyi SUN
- Publication Type:Journal Article
- Keywords:
bone marrow-derived mesenchymal stem cells(BMSCs);
chondrocytes;
coculture;
fullydeproteinized bone(FDB);
articular cartilage defect;
repair
- From:
Orthopedic Journal of China
2006;0(16):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To investigate the rlpairing effect on articular cartilage defects by composite of cocultures of autogenic bone marrow-derived mesenchymal stem cells(BMSCs) and chondrocytes with allogenic fully deproteinized bone(FDB),in order to provide basis for optimizing seeding cells resources.[Method]Seeding cells were collected from two-passaged BMSCsand chondroeytes and then cocultured at the rate of 2 to 1.Full thickness articular cartilage defects in the knee joints of rabbits repaired by cocultured cells seeded into allogenic FDB were served as experimental group A,by simple FDB as control group B and by nothing as blank control group C.Repaired tissues were evaluated with macroscopic views,histological scores and immunohistochemistrical stains at 8 and 16 weeks postoperatively.[Result]Chondrocytes cocultured riched in extracellular matrix and proliferated promptly.In A regenerated tissues represented hyaline-like,smoothness and flat.In group B and C,repaired tissues were fiberous and no repaire in group C.Histological scores of experimental group A excelled group B and C with statistically significant differences(P0.05).Immunohistochemistrical stains showed that cells in the zones of repaired tissues were larger in size,arranged columnnedly,riched in type-Ⅱ collagen matrix and integrated satisfactorily with native adjacent cartilages and subchondral bones in the experimental group A at 16 weeks postoperatively.[Conclusion]Cocultures of autogenic BMSCS with chondrocytes can promote proliferation of chondrocytes and production of chondral matrix.Cocultures as seeding cells can save a number of chondrocytes,shorten culturing periods and reduce subcultured times.Cocultures embedded into FDB can repair articular cartilage defects effectively.
