Tumor necrosis factor alpha expression during progression of left ventricular remodeling in rat myocardial infarction model
- VernacularTitle:TNF?在大鼠心肌梗死后心室重构进程中表达的变化及意义
- Author:
Shuiming GU
- Publication Type:Journal Article
- Keywords:
myocardial infarction;
ventricular remodeling;
tumor necrosis factor
- From:
Academic Journal of Second Military Medical University
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the changes of tumor necrosis factor ?(TNF?) expression and the relationship between TNF? and cardiac function during the progression of left ventricular remodeling in an experimental model of rat myocardial infarction(MI). Methods: Ninety male Sprague Dawley rats were randomly divided into sham-operated and MI groups. Rats in MI group were induced by ligation of the anterior descending coronary artery and were subdivided into 1 d,7 d,14 d,28 d and 42 d groups. Sham-operated animals underwent the same surgical procedure without ligation. Heart function and left ventricular remodeling were studied by echocardiography and hemodynamics. The expression of TNF? mRNA was assessed by reverse transcription-polymerase chain reaction. Results: From day 14 after operation,left ventricular end-diastolic diameter(LVEDD) began to increase in MI group,and fractional shortening(FS) and left ventricular ejection fraction(LVEF) began to decrease; both the increase and decrease reached the maximal on day 42 after operation. From day 1 after operation,left ventricular end-diastolic pressure(LVEDP) began to increase, and left ventricular(LV) pressure maximal rate of rise and fall (dp/dt_(max) and (dp/dtmin)) began to decrease; both increase and decrease reached the maximal on day 42 after operation. TNF? began to express on day 1 after MI, reached peak on day 7, and began to decrease thereafter; there was significant differences between MI group and sham-operated group.The expression of TNF? was positively correlated with LVEDD(r=0.94,P
