A novel beta-glucan produced by Paenibacillus polymyxa JB115 induces nitric oxide production in RAW264.7 macrophages.
10.4142/jvs.2009.10.2.165
- Author:
Zhi Qiang CHANG
1
;
Joong Su LEE
;
Mi Hyun HWANG
;
Joo Heon HONG
;
Hee Kyoung JUNG
;
Sam Pin LEE
;
Seung Chun PARK
Author Information
1. College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea. parksch@knu.ac.kr
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords:
beta-glucan;
macrophages;
nitric oxide;
Paenibacillus polymyxa
- MeSH:
Animals;
Bacillus/*metabolism;
Cell Line;
Cyclooxygenase 2/biosynthesis/genetics;
Interleukin-6/biosynthesis/genetics;
Lipopolysaccharides/pharmacology;
Macrophages/*drug effects/enzymology/immunology;
Mice;
Nitric Oxide/*biosynthesis/immunology;
Nitric Oxide Synthase Type II/biosynthesis/genetics/metabolism;
RNA, Messenger/biosynthesis/genetics;
Reverse Transcriptase Polymerase Chain Reaction;
beta-Glucans/metabolism/*pharmacology
- From:Journal of Veterinary Science
2009;10(2):165-167
- CountryRepublic of Korea
- Language:English
-
Abstract:
The effect of extracellular beta-(1-->3), (1-->6)-glucan, produced by Paenibacillus polymyxa JB115, on nitric oxide (NO) production in RAW264.7 macrophages was investigated. beta-glucan induced the production of NO by RAW264.7 macrophages in a concentration- and time-dependent manner. Moreover, beta-glucan stimulation increased the mRNA expression of iNOS, COX-2 and IL-6 in RAW264.7 macrophages in a concentration-dependent manner.
