Influence of Wt-p53 gene by adenovirus vector on proliferation of keloid fibroblasts
- VernacularTitle:腺病毒介导的wt-p53基因对人瘢痕疙瘩成纤维细胞增殖的影响
- Author:
Zhibo XIAO
;
Lijun HAO
;
Lihong REN
;
Wen TENG
- Publication Type:Journal Article
- Keywords:
Wt-p53 gene;
Fibroblasts;
Proliferation
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study wt-p53 gene's influence on the proliferation of keloid fibroblasts in vitro. Methods wt-p53 gene was transfected into keloid fibroblasts by adenovirus vector. wt-p53 mRNA was analyzed by RT-PCR; wt-p53 protein was evaluated by indirectiy immunofluorescence; The ability of proliferation of keloid fibroblasts was analyzed by cell growing curves; The cell cycle of KFB was checked by FCAS. Results The expression of wt-p53 mRNA and protein was obviously higher in the fibroblasts of the experimental group than those of control group; the rate of G_0~G_1 in cell cycle was higher in the fibroblasts of the experimental group than those of control group; at the same time, the rate of G_2~M was lower in fibroblasts of the experimental group than those of control group (P
