The expression of NOV and WT1 genes in renal cell carcinoma:a quantitative RT-PCR analysis
- VernacularTitle:肾母细胞瘤过表达基因和WT1基因在肾癌组织中转录水平的定量研究
- Author:
Zhihong NIU
;
Jiaju LU
;
Kejia DING
;
Shanjun LI
- Publication Type:Journal Article
- Keywords:
Kidney neoplasms;
Carcinoma;
Genes
- From:
Chinese Journal of Urology
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the quantitative expression of mRNA levels of human NOV and WT1 genes in renal cell carcinoma ( RCC). Methods Using quantitative real-time RT-PCR analysis, we quantified NOV and WT1 mRNA levels in the samples from 57 patients (40 men and 17 women; mean age,65 years) with RCC. Of them,39 cases had T1 stage tumor, 13 had T2 stage and 5 had T3a stage by UICC pathologic staging;and 14 cases had G1 tumor,23 had G2 and 20 had G3 by Fuhrman grading. The expression levels of NOV and WT1 genes,and their association were analyzed. Results The median level of NOV mRNA expression was significantly lower in RCC ( 1. 17) than in normal kidney tissue (4. 32, P
