The effect of irbesartan on the expression of angiotensin-converting enzyme 2 mRNA in kidney of spontaneously hypertensive rats
- VernacularTitle:伊贝沙坦对自发性高血压大鼠肾脏血管紧张素转换酶2基因表达的影响
- Author:
Liu YANG
;
Changhui LIU
;
Yupeng BAI
- Publication Type:Journal Article
- Keywords:
Rats, inbred SHR;
Renin-angiotensin system;
Peptidyl-depeptidase A;
Angiotensin II;
Angiotensin II type 1 receptor blockers
- From:
Chinese Journal of Geriatrics
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the regulation effect of irbesartan on the expression of angiotensin-converting enzyme 2 (ACE2) mRNA in the kidney of spontaneously hypertensive rats (SHR). Methods Twenty 14-week-old male SHR were randomly divided to SHR group (n=10) and irbesartan group (n=10); ten 14-week-old male WKY group were served as control. Irbesartan group was given a daily dose of 50 mg/kg irbesartan for 12 weeks by gavage and the other two groups were fed with a normal diet. ACE2 in kidney was examined by immunohistochemistry and RT-PCR. Plasma angiotensin II in each group were estimated by radioimmunoassay (RIA). Results The ACE2 mRNA expression was significantly down-regulated in the kidney of SHR group compared with WKY group (0. 72?0. 11 vs 1. 11?0. 15). After 12 weeks treatment with irbesartan, the attenuated ACE2 expression in irbesartan group was markedly enhanced compared with SHR group (1. 03?0. 13 vs 0. 72?. 0. 11). ACE2 mRNA expression in SHR kidney was correlated significantly with plasm angiotensin II level. (r = 0. 83, P
