Biological responses of osteoblast-like cells in different maturation stages under shear flow
- VernacularTitle:不同分化程度的类成骨细胞对流体刺激的生物反应
- Author:
Dajiang DU
;
Zhongli GAO
;
Guangyao LIU
- Publication Type:Journal Article
- Keywords:
mechanical stimulation;
osteogenic differentiation
- From:
Orthopedic Journal of China
2006;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To sturdy the biological responses of osteoblast-like cells in different maturation stages under shear flow.[Method]Flow chamber system was used to apply shear flow stimulation of 4.62dyn?s/cm~2 for 2 hours on osteoblast-like cell MC3T3-E1 of different maturation stage induced by osteogenic media,and then samples for dsDNA content and ALP activity analysis were collected.[Result]On 24 hours after simulation,there was not obvious change of ALP activity with non-osteogenic inducing group,an increase was observed in the group of 4 days of differentiation,while an decrease in the group of 8 days of differentiation.[Conclusion]Biological responses of osteoblast-like cells in different maturation stages under shear flow is quite different,and this may be an important role in bone growth and reconstruction.
