Experimental study on treatment of acute spinal cord injury by autologous activated Schwann cells transplantation in the rat
- VernacularTitle:自体激活雪旺细胞移植治疗急性脊髓损伤的实验研究
- Author:
Shiqing FENG
;
Xianhu ZHOU
;
Xiaohong KONG
- Publication Type:Journal Article
- Keywords:
Schwann cells;
Cell transplantation;
Spinal cord injuries;
Axons
- From:
Chinese Journal of Orthopaedics
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of treating acute spinal cord injury in rats by transplantation of autologous activated Schwann cells(AASCs). Methods Unilateral saphenous nerves were ligated directly, free it and culture Schwann cells 1 week later using the tissue clot method. Nerve growth factor(NGF) and brain-derived neurotrophic factor(BDNF) in medium were detected in different periods. 90 female Wistar rats[(200?30) g] were randomly assigned to 3 different study groups as follows: control group A(n=30): 20% DMEM injection; research group B(n=30): autologous Schwann cells(ASCs) transplantation; research group C (n=30): AASCs transplantation. The cells were purified before transplantion to the injuried T10 spinal cord site of rats (New York University type weight drop apparatus, NYU). The recoveries of the lower extremity were observed using Basso-Beattie-Bresnahan(BBB) locomotor scoring system and somatosensory evoked potential and motor evoked potentials(SEP & MEP). And then observe the coticospinal tract(CST) using the biotinylated dextran amine(BDA) tracing. Results BBB score was higher in research group than the control group 4 weeks after injury, the statistical difference was significant(P
