The Effects of rhG-CSF on morphology,phenotype and function of neutrophil in patients with malignant lymphoma after the treatment of chemotherapy
- VernacularTitle:恶性淋巴瘤患者化疗后应用rhG-CSF对中性粒细胞的影响
- Author:
Jihua ZHONG
- Publication Type:Journal Article
- Keywords:
rhG-CSF;
lymphoma;
neutrophil;
morpholo-gy;
function;
phenotype
- From:
China Oncology
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Background and purpose:Recombinant human granulocyte colony-stimulating factor(rhG-CSF)is a kind of polypeptide.It can stimulate the haemopoietic stem cell and granule progenitor cell to differentiate and proliferate.The neutrophil can be accelerated to release and promote its function of phagocytosis,chemotaxis and oxidative burst by rhG-CSF.In this study,we aimed to explore the effect of rhG-CSF on morphology、phenotype and function of neutrophil in patients with malignant lymphoma after treatment of chemotherapy.Methods:White blood cell(WBC) was counted by the method of COULTER.The morphology of neutrophil was observed on Wright's-stained peripheral blood smear.The phagocytotic function and chemotaxis of neutrophil were assessed by either measuring the amount of hydrogen peroxide or by the agarose method.Oxidative burst and phenotype of neutrophil was analysed by flow cytometry using immunofluorescence technique.Results:①There was Hypogranular in some neutrophilic cytoplasm after chemotherapy,the number of "toxic" granulation,vacuoles and Dohle bodies increased from 5.4%,3.2%,0.5% to 55.0%,11.8%,4.1% in neutrophils from patients with malignant lymphoma after rhG-CSF administration,.②Compared to normal control,5.76?mol/LH_(2)O_(2).10~(-6)PMN,1.85,76.4,the neutrophil functions of phagocytosis,chemotaxis,oxidative burst were impaired after chemotherapy 2.73?mol/LH_(2)O_(2).10~(-6)PMN,0.97,51.8%,rhG-CSF increased the function,7.02?mol/LH_(2)O_(2).10~(-6)PMN,1.89,78.4%,the neutrophil function from these patients had returned to normal or were even exceeded.③ A mild increase of CD64 expression from 5.4%,3.2%,0.5% and a significant decrease of CD62L expression from 7.47% to 3.95% were found in patients after rhG-CSF treatment.No changes of CD16,CD32,CD14 and CD11b expression were detected in these patients before or after G-CSF administration.Conclusions:rhG-CSF administration can modify the morphology,phenotypeand partially recover the impaired function of neutrophil from the patients with Malignant Lymphoma after chemotherapy,andit may improve anti-infection ability of the human body.
