siRNA-2 can enhance the drug-sensitivity of HL-60 cells to homoharringtonin
- VernacularTitle:siRNA-2提高HL-60细胞对高三尖杉酯碱敏感性的实验研究
- Author:
Haiyan HU
;
Yuan ZHANG
;
Ping LI
;
Meng XU
- Publication Type:Journal Article
- Keywords:
siRNA;
bcl-2 gene;
Drug-sensitivity;
HL-60 cell;
Homoharringtonin
- From:
Cancer Research and Clinic
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of siRNA targeting bcl-2 gene on the drug-sensitivity of HL-60 cell to homoharringtonin(HT). Methods siRNA, selected by previous experiments and packaged with lipofectamine 2000, was transferred into HL-60 cells. Six hours later, the cells were cultured with homoharringtonin. The cell growth of HL-60 cells was detected by MTT methods at 24, 48, 72 hours respectively. The level of bcl-2 protein and ROS (reactive oxygen species, ROS) as well as membrane potential of mitochondrion was determined by flowcytometry. Results The siRNA could significantly increase the inhibitive effect of homoharringtonin on growth of HL-60 cells. The combination of siRNA with homoharringtonin resulted in the falling of bcl-2 protein level and rising of ROS level as well as descending of membrane potential of mitochondrion of HL-60 significantly (P
