The co-culture system of MSCs and injured PC12 in vitro could inhibit the apoptosis of PC12
- VernacularTitle:骨髓基质干细胞与受损PC12细胞的共育体系减少PC12的凋亡
- Author:
Jin ZHOU
;
Xiaoguang LUO
;
Chaodong ZHANG
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cells;
PC12 cells;
Amyloid beta-protein;
Fibroblast growth factor 2;
Apoptosis
- From:
Chinese Journal of Neurology
2005;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the co-culture system of marrow stromal cells (MSCs)and A?1-40 injured PC12 in vitro and to evaluate the effect and mechanisms of the system inhibiting apoptosis of PC12 induced by A?1-40.Methods MSCs and PC12 were cultured in vitro and identified by CD44 immunofluorescent staining;PC12 were damaged by A?1-40,and transferred by transwell followed by the classification into 5 groups. PI and Annexin-V co-fluorescent staining was performed,then PC12 apoptosis were detected by flow cytometry and EM;Supernatant was analyzed by enzyme-linked immunoadsordent assay (ELISA) to detected TGF-?,NGF,BDNF,and bFGF. Results About 96% MSCs showed CD44 positive cells. Co-culture group had the lowest rate of PC12 apoptosis(46.17%?8.28%,F=61.637,P0.05). Conclusion The co-culture system of MSCs and A?1-40 injured PC12 in vitro could inhibit apoptosis of PC12 induced by A?1-40. Thus grafted MSCs have the possibility to inhibit neuronal apoptosis by A?in the diseased brain.
