Comparison of expression of renal transforming growth factor-?_1 and its receptors in rats treated by(cyclosporine) A and tacrolimus
- VernacularTitle:他克莫司和环孢素A对大鼠肾脏转化生长因子及其受体表达影响的比较
- Author:
Maode LI
;
Sheng HE
;
Bing PENG
- Publication Type:Journal Article
- Keywords:
FK506;
Cyclosporines;
Transforming growth factor beta;
Receptors,transforming growth factor beta
- From:
Chinese Journal of Organ Transplantation
2005;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the expression of renal transforming growth factor-?_1(TGF-?_1),its receptors type Ⅰ and Ⅱ(T?RⅠ and T?R Ⅱ) in two rat models of chronic nephropathy induced by CsA and FK506 respectively.Methods Rat models of chronic CsA-/FK506-induced nephropathy were established by administering sandimun Neoral and Prograf separately.Then their kidneys were dissected and the protein expression of TGF-?_1,T?RⅠand T?RⅡ,the mRNA levels of T?RⅠ and T?RⅡ was detected by using immunohistochemistry(IHC) and in situ hybridization(ISH) respectively.Results The IHC revealed that the integrated optical densities(IODs) of renal TGF-?_1,T?RⅠ and T?RⅡ were(605.24)?(140.24),(876.28)?(208.73) and(981.59)?(258.65) respectively in CsA-treated rats,(488.37)?(101.76),(586.63)?(131.21) and(543.34)?(105.26) respectively in FK506-treated ones.The ISH indicated that the IODs of renal T?RⅠ and T?RⅡ mRNA were(905.08)?(158.75) and(1090.92)?(242.73) respectively in CsA-treated rats,(661.37)?(205.65) and(716.27)?(195.55) respectively in FK506-treated ones.The difference of the above-mentioned five factors between the two groups was significant(P
