Effect of removing stasis medicinal herbs on proliferation and proteomics of THP-1 cell line induced by endotoxin
- VernacularTitle:活血化瘀中药对内毒素诱导THP-1细胞增殖及蛋白质组的影响
- Author:
Cui ZHANG
;
Peixun WANG
;
Lian ZHOU
;
Yan DONG
- Publication Type:Journal Article
- Keywords:
Removing stasis medicinal herbs;
THP-1;
Two-dimensional eletrophoresis;
Proteomics
- From:
Chinese Journal of Immunology
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the proteomics of blood-activating and stasis-removing(BASR) herbs in cellular model of endotoxin infection in vitro.Methods:Cellular model of endotoxin was induced by LPS,and a technical system by two-dimensional electrophoresis was widely used,proteins in different expression were analyzed by PDQust 7.1.1.Results:There were 176 protein spots in model group,in which 26 protein spots with different expression were sieved.Compared the protein profiles of TanshinoneⅡA group in model group,proteins were found as two-way-regulated.Among them,50% proteins were observed down-regulated and 38.5% proteins were seen up-regulated in TanshinoneⅡA group.11.5% more up-regulated.Conclusion:Cellular model of endotoxin induce was applied to sieving traditional Chinese medicines in laboratory;The Tanshinone ⅡA can inhibit the proliferation of THP-1 cell line in infection model,and can modify expression of some proteins.
