Effect and mechanism of IL-18BP on acute graft-versus-host disease
- VernacularTitle:IL-18BP对急性移植物抗宿主病的影响及机制研究
- Author:
Min XU
- Publication Type:Journal Article
- Keywords:
IL-18BP;
Th1/Th2;
Acute graft-versus-host disease;
Mice
- From:
Chinese Journal of Immunology
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect and the mechanism of interleukin-18 binding protein(IL-18BP) on acute graft-versus-host disease(aGVHD) in allogeneic bone marrow transplantation(allo-BMT) in mice.Methods:C57BL/6 recipient mice irradiated with 60Co were randomly divided into 2 groups: group A and B and were transfused with BALB/c donor cells.After transplantation recipient mice in group A were treated with NS subcutaneously daily from day 0 to day 5 and recipient mice in group B with 1 ?g IL-18BP,separately.The severity of aGVHD was evaluated in the two groups.Concentrations of IFN-?,IL-2,IL-4 and IL-10 were measured using enzyme-linked immunosorbent assay(ELISA).Results:aGVHD response was more severe in group A than that in group B.The serum concentrations of IFN-? and IL-2 in group A were higher than that in group B,while the serum concentrations of IL-10 and IL-4 were lower than that in group B.Conclusion:①IL-18BP can decrese aGVHD response;②That IL-18BP can promote the differentiation of CD4~+T cells into CD4~+Th2 cells,that is promoted by IL-18BP might be one of the mechanisms of decreasing aGVHD.
