RADIOIMMN OASSAY OF ARGININE VASOPRESSiN IN UNEX-TRACTED PLASMA
- VernacularTitle:未经提取的血浆中精氨酸加压素的放射免疫测定
- Author:
Guangmu LI
- Publication Type:Journal Article
- Keywords:
neuropeptide;
arginine vasopressin;
radioimmunoassay
- From:
Academic Journal of Second Military Medical University
1981;0(03):-
- CountryChina
- Language:Chinese
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Abstract:
A specific radioimmunoassay (RIA) for arginine vasopressin (AVP) in unex-tracted human and rat plasma was developed by using an AVP antiserum prepared by ourselves.The sensitivity was 1.2pg per tube, and the recovery of AVP was 112% for human plasma and 117 % for rat plasma. Serial dilutions of plasmay ielded curves that were parallel to the AVP standard curve The intraassay and interassay coefficients of variation were 4.2% and 23.04%, respectively.By using this method, the mean concentrations of AVP were 5.2pg/ml in 4 normal human subjects and 4 2?0.3pg/ml (x?S.E.)in 10 intact rats.The value of AVP in the rat plasma reached 34.4? 9.7pg/ml (x?S.E.)after 36-hour dehydration.We believe that this method can reflect changes of AVP concentration under pathophysiological conditions, and can be used as a useful tool in the diagnosis and investigation of some diseases
