Intervertebral disc cell therapy for regeneration:mesenchymal stem cell cultivation in vitro and intervention in TGF-?1 implantation in rabbit intervertebral discs
- VernacularTitle:TGF-?1干预下体内兔骨髓间充质干细胞对退变椎间盘治疗的实验研究
- Author:
Ziru ZHAO
;
Xiaotao WU
;
Yabin QI
- Publication Type:Journal Article
- Keywords:
Marrow mesenchymal stem cells(MSCs);
Inter vertebral disc;
TGF-?1;
Nucleus pulposus cell
- From:
Orthopedic Journal of China
2006;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To evaluate whether transplanted marrow mesenchymal stem cells interfered in TGF-?1 can differentiate to nucleus pulposus cells and increase the amount of proteoglycan and collagenase Ⅱ content in intervertebral discs.[Method]We used an in vivo model to investigate the feasibility of marrow mesenchymal stem cells that cultured in vitro and interfered in TGF-?1 delivery,retention,and survival in the degeneratived disc space.In 2,4,6,8 weeks we used immunohistochemical staining to determine the change of collagenase Ⅱ content;spectrophotometry to determine the change of amount of proteoglycan with Phlorglucinol;the experiment date were analyzed by SPSS 11.5 soft ware.[Result]We found MSCs could maintain viability and proliferate within the rabbit inter vertebral disc.The amount of proteoglycan and collagen Type Ⅱ content of the intervertebral in matrix synthesis in the experiment group was increased in 8 weeks.We found no changes in the modle group.[Conclusion]Our data suggest that transplanted marrow mesenchymal stem cells in vivo can survive and increase proteoglycan and collagen Type Ⅱ amount interfered in TGF-?1 in some periods,which support its potential use as a treatment of intervertebral disc degeneration.
