Expression of costimulatory molecule B7-1 and B7-2 in warm ischemia/reperfusion(I/R) injury of liver in rats
- VernacularTitle:共刺激分子B7-1、B7-2在大鼠热缺血再灌注肝脏表达意义的研究
- Author:
Zhipeng JI
;
Kesen XU
;
Yi LIU
- Publication Type:Journal Article
- Keywords:
Costimulatory molecule?Reperfusion(injury)?Liver Transplantation?Rats,Wistar
- From:
Chinese Journal of Current Advances in General Surgery
1999;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of costimulatory molecule B7-1 and B7-2 by warm ischemia/reperfusion(I/R)injury in rat liver and the meaning of immunology.Methods:Male Wistar rats were randomly divided into 3 groups:A(ischemia 30 mins,referfusion 24h),B(ischemia 60 mins,referfusion 24h),C(sham operation group).A model of hepatic warm I/R in rats was established according to the method of Ohmorid.The expression of B7-1 and B7-2 mRNA was analyzed by real-time reverse transcription polymerase chain reaction(RT-PCR).Results:Compared with the sham operation group,the expression of B7-1 and B7-2 mRNA increased significantly in the rats undergoing I/R procedure(P
