SOME INTERFERENCE IN THE ASSAY OF VITAMIN E IN BIOLOGICAL MATERIALS
- VernacularTitle:荧光法测定生物样品中维生素E的某些干扰因素
- Author:
Youping HE
;
Lianzhen ZHU
- Publication Type:Journal Article
- From:
Acta Nutrimenta Sinica
1956;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
In this work, some interference in the assay of vitamin E in liver and serum was studied and the method was improved.The sample must be protected from oxidative attack. The interference of high vitamin A content in liver could be removed by H2SO4. The amount of KOH for saponification must be in keeping with the amount of sample assayed both in synthetic a-tocopherol and in biological material by fluorometry. A linear correlation of the fluorescence and the vitamin E content was obtained when 0.5ml of 10 N KOH was used for the saponification of 0.5-100?g of ?-tocopheryl acetate.
