Sodium butyrate up-regulate CD86 molecule on acute leukemia cells partially dependent CREB
- VernacularTitle:丁酸钠部分依赖CREB上调急性白血病细胞CD86分子
- Author:
Wei LI
;
Xiaofeng CHEN
;
Guanjun WANG
- Publication Type:Journal Article
- Keywords:
Sodium butyrate;
AUT gel electrophoresis;
CREB;
Antigen;
CD86
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effects of sodium butyrate on the expression of CD86 molecule on acute leukemia cells and explore the mechanisms of action.Methods:The expression of CD86 in NB4,HL-60 and U937 treated by SB or not was assayed by flow cytometric analysis.The alteration of CD86 mRNA was examined by semiquantitative RT-PCR.AUT gel electrophoresis was applied to check the state of histone acetylation.The content of phospho-CREB was assayed by pCREB kit.Results:Up-regulation of CD86 was observed on those cells treated by SB.The levels of CD86 mRNA in SB treated cells were statistically enhanced.The acetylation degrees of SB treated cells were higher than control groups.The contents of phospho-CREB were also raised in SB treated cells.Conclusion:SB can improve the acetylation states of acute leukemia cells,remodel the chromatin which contributes to the binding on DNA of transcription factors,such as CREB and then promote the transcription and expression of CD86.
