Effect of retinoic acid on the secretion of C3 and factor B in lung carcinoma cell line A549 induced by TNF-? and IL-6
- VernacularTitle:视黄酸对TNF-?和IL-6诱导的肺癌细胞分泌C3和B因子的影响
- Author:
Yuxia ZHAO
;
Guang LI
;
Runjiang YU
- Publication Type:Journal Article
- Keywords:
Retinoic acid;
Complement;
Lung cancer cells
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of retinoic acid(RA) which is used in malignant tumor therapy on the secretion of C3 and factor B in human lung cancer cell line A549 induced by TNF-? and IL-6.Methods:C3 and factor B in the culture medium were measured by ELISA and Western blot,the mRNA expression of C3 and factor B was analyzed by RT-PCR.Results:When using TNF-?(10 ?g/L),IL-6(50 ?g/L) and RA(1 ?mol/L),the results of ELISA,Western blot and RT-PCR showed that TNF-? and IL-6 promoted A549 cells excreting C3 and factor B and their mRNA expression;RA alone did not affect C3 and factor B;in TNF-? group and TNF-?+RA group,the amounts of C3 were 91.40?12.59 and 133.59?11.25(ng/10~6 cells) respectively(P0.05),and the amounts of factor B were 13.07?2.50 and 32.89?4.22(ng/10~6 cells) respectively(P
