Effect of carboxymethyl-chitosan on proliferation and collagen synthesis in keloid fibroblasts in vitro
- VernacularTitle:羧甲基壳多糖对瘢痕疙瘩成纤维细胞增殖和胶原合成的影响
- Author:
Hairong CHEN
;
Chunxia LI
;
Xiguang CHEN
;
Yanfang WU
- Publication Type:Journal Article
- Keywords:
Carboxymethyl-chitosan;
Keloid fibroblast;
Proliferation;
Collagen synthesis
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of carboxymethyl-chitosan (CM-CH) on keloid fibroblasts (KFB) proliferation and collagen synthesis. Methods Keloid fibroblasts were isolated from fresh keloid tissue and cultured. The effect of CM-CH on proliferation was examined by MTT assay. The synthesis of collagen was evaluated by hydroxy proline (HP) colorimetric analysis. Results The fibroblasts were treated with CM-CH at concentration of 10 ?g/ml, 50 ?g/ml, and 100 ?g/ml 48h and 72h after treatment, and all of the three concentrations showed inhibitory effects significantly (P0.05) at 200 ?g/ml concentration after 24h, 48h, and 72h. CM-CH at concentration of 10 ?g/ml, 50 ?g/ml, and 100 ?g/ml after treatment for 48h on KFB could markedly decrease the synthesis of collagen (P
