Study of VEGF and BMP-2 genes expressions inside the necrotic rabbit femoral head
- VernacularTitle:激素相关性股骨头坏死股骨头内VEGF和BMP-2的表达
- Author:
Hongbin ZHAO
;
Linzhi LI
;
Shihe LI
- Publication Type:Journal Article
- Keywords:
Femoral head necrosis;
Rabbit model;
VEGF;
BMP-2;
Immunohistochemistry;
In situ hybridization;
Real-time quantitative PCR
- From:
Orthopedic Journal of China
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To study the dynamic changes of the VEGF and BMP-2 genes expressions inside the necrotic femoral head of rabbits.[Method]Made rabbit model of femoral head necrosis(FHN) with the method of twice intravenous injection with low dosage lipo polysaccharide(LPS)(10 ?g/kg body weight,at a time interval of 24 hours.)and 3 times intramuscular injection with methylprednisolone(MPS)(20mg/kg body weight,at a time interval of 24 hours.().)Immunohistochemistry,in situ hybridization and extract total RNA for real-time quantitative PCR were performed and successfully designed the suitable probes and primerswas used to detect the dynamic changes of the VEGF and BMP-2 genes expressions inside the femoral head of rabbits.[Result]The expressions of the VEGF and BMP-2 genes in group B(model group)at the 4,8,12 th and 16 th week were negative and real-time quantitative PCR showed that the level of the VEGF and BMP-2 mRNA genes expressions were obviously lower than that of control group.[Conclusion]The VEGF and BMP-2 genes expressions are inhibited in FNH model induced by LPS and MPS.
