Repair of autogenous calvarial defects by marrow stromal cells/ceramic bovine bone/hydrogel complex
- VernacularTitle:陶瓷化骨/水凝胶与骨髓基质细胞修复自体颅骨缺损实验
- Author:
Dawei HE
;
Yan JIN
;
Kai LUO
- Publication Type:Journal Article
- Keywords:
Tissue engineering;
Bone;
Ceramic bovine bone/hydrogel
- From:
Journal of Practical Stomatology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the feasibility of marrow stromal cells/ceramic bovine bone/hydrogel in the repair of autogenous calvarial defects. Methods:The complex of ceramic bovine bone,hydrogel,rhBMP-2,TGF-? and marrow stromal cells(MSCs) induced by minerlization inducing medium was prepared and applied in the repair of autogenous calvarial defects in 8 SD rats(group A).Ceramic bovine bone /MSCs was used as control in another 8 SD rats(group B). 4 and 8 weeks after operation 4 rats in each group were sacrificed, the osteogenesis was examined by HE staining,modified Mallory's trichrome staining and image analysis.Results:4 weeks after operation, osseous plerosis can be seen in both groups, the quantity of bone-like tissues formation(?m~2) in group A and group B was 6 813.09?96.32 and 3 839.25?104.52(P
