Relationship between the expression of EGFR,C-erbB-2 proteins and the lung metastatic potential of salivary adenoid cystic carcinoma cell lines
- VernacularTitle:EGFR及C-erbB-2蛋白表达与腺样囊性癌细胞系转移力之间的关系
- Author:
Jie MA
;
Ming ZHONG
;
Zhaoyuan WANG
- Publication Type:Journal Article
- Keywords:
Epidermal growth factor receptor;
C-erbB-2 protein;
Adenoid cystic carcinoma;
Lung metastasis
- From:
Journal of Practical Stomatology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expressions of epidermal growth factor receptor(EGFR) and C-erbB-2 in two salivary adenoid cystic carcinoma cell lines with different metastatic potential. Methods:Salivary adenoid cystic carcinoma SACC-83 cell line and its lung metastatic cell line SACC-LM were cultured in vitro, their cytoplasm and membrane samples were extracted, and the expressions of EGFR and C-erbB-2 were detected by Western blot, the results were quantitatively analyzed by FluorChem V2.0 software.Results:EGFR expression level in cell membrane was higher than that in cytoplasm(P0.05) in both cell lines.Conclusion:Higher EGFR expression in cytoplasm and higher C-erbB-2 expression may be related to the metastatic potential of SACC-LM cells.
