Difference of the migration ability between PTEN transfected breast cells and non-transfected cells
- VernacularTitle:乳腺癌细胞转染野生型PTEN质粒后转移能力的变化
- Author:
Guanping LIN
;
Jinwen HUANG
;
Keyuan ZHOU
- Publication Type:Journal Article
- Keywords:
PTEN gene;
Breast cancer cells;
Migration
- From:
Cancer Research and Clinic
1999;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To disclose the influence of PTEN on the migration of breast cancer cells ZR-75-1. Methods Wild-type PTEN gene was transtected into ZR-75-1 breast cancer cells which lack PTEN gene, tranfected cells were selected by puromycin and the protein encoded by PTEN gene was tested by Western-blot. The inhibition rate of invasion and adhesion of ZR-75-1 was tested on reconstituted basement membrane. Results Wild-type PTEN gene was successfully transfected into ZR-75-1 and expressed efficiently. The inhibition rate of invasion and adhesion is 70.4 % and 60.0 % respectively. Conclusion PTEN gene can restrict the migration of breast cancer cells in some degree, so whether PTEN gene is deleted or not can partly estimates the risk of migration of breast cancer cells.
