The expression and significance of the role of differentially expressed gene interleukin 8 of early squamous cervical carcinoma
- VernacularTitle:早期宫颈鳞癌差异表达基因IL-8的表达及意义
- Author:
Lihuan CUI
;
Haixia SHANG
;
Jun XIE
;
Suhui WU
- Publication Type:Journal Article
- Keywords:
Interleukin-8;
Lymph node metastases;
Uterine squamous cervical carcinoma
- From:
Cancer Research and Clinic
1999;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the correlation between the expression of interleukin 8(IL-8) and lymph node metastases in earlier uterine squamous cervical cancer and to study the mechanism of metastases of earlier squamous cervical cancer. Methods IL-8 mRNA expression was examined with reverse transcription polymerase chain reaction(RT-PCR) and tissue microarray to determine the expression of IL-8 protein. The matrix metalloproteinase 9( MMP-9) protein and microvessel density (MVD) in earlier squamous cervical cancer were decided respectively by antibody against IL-8, MMP-9 and CD-34 associated antigen and Immunohistochemical analysis. Results The cervical cancer with lymph node metastases express IL-8, MMP-9 and MVD at significantly higher level than negtive metastatic lymph node(P =0.00,0.00,0.02). Otherwise, the express of IL-8 correlated strongly and positively with MVD of cervical cancer(r =0.251,P
