Role of Tumor Necrosis Factor-Alpha Monoclonal Antibody in Cell Apoptosis after Combined Liver and Kidney Transplantation in Rats
- VernacularTitle:抗TNF-?单克隆抗体对大鼠肝肾联合移植术后肝肾细胞凋亡的影响
- Author:
Shuling HAN
;
Lixin YU
- Publication Type:Journal Article
- Keywords:
Combined liver and kidney transplantation Cell apoptosis Tumor necrosis factor-alpha Monoclonal antibody
- From:
Chinese Journal of Bases and Clinics in General Surgery
2003;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the potential role of tumor necrosis factor-alpha (TNF-?) in apoptosis after combined liver and kidney transplantation in rats. Methods Eighty rats which had combined liver and kidney transplantation were randomly paired, were divided into study group (n=20) and control group (n=20). 40 ml of 4 ℃ sodium chloride and anti-TNF-? monoclonal antibody (30 ml was infused from portal veins to donated livers and 10 ml from renal arteries to donated kidneys) were infused to the study group (0.1 mg/kg weight),and the same quantity of 4 ℃ sodium chloride was infused the control group. Venous blood was drew at different phases after the transplantations to detect the function of kidney and liver. The level of TNF-? and the cell apoptosis were detected in the transplanted tissues of liver and kidney by ELISA and terminal deoxynucleotidy transferase mediated dTUP-biotin nick-end labeling (TUNEL). Results The levels of AST, ACT, Cr and BUN in the study group were significantly lower than those of the control group at the same phases (P
